Stroehmann I, DeWitt C W
Immunology. 1972 Dec;23(6):929-35.
Lymphoid cells of the dark agouti (AgB 4 or Rt H1.1) rat were extracted with 3M KCl. This soluble extract was shown by inhibition of specific, cytotoxic alloantisera to contain the private antigen, AgB 4, and the public antigens, AgB 26, 30 and 29. The public antigens AgB 27 and 28 were not assayed. A species-specific antigen and a non-AgB antigen were also present. Equal concentrations of the various antigens were not obtained. Gel filtration of this extract over Sephadex G-200 yielded AgB 4,26, and 30 and the species antigen at the front of the inner bed volume with a minor AgB 4 peak at about 65,000 mol. wt. AgB 29 and the non-AgB antigen were not recovered. As AgB 29 was present in high concentration in the extract and shown not to be highly labile, it is suggested that it may be present on a molecule distinct from that carrying AgB 4,26 and 30.
用3M氯化钾提取深色刺鼠（AgB 4或Rt H1.1）大鼠的淋巴细胞。通过抑制特异性细胞毒性同种异体抗血清表明，这种可溶性提取物含有私有抗原AgB 4以及公共抗原AgB 26、30和29。未检测公共抗原AgB 27和28。还存在一种物种特异性抗原和一种非AgB抗原。各种抗原未获得相等浓度。将该提取物在Sephadex G - 200上进行凝胶过滤，在内床体积前沿得到AgB 4、26和30以及物种抗原，在约65,000分子量处有一个较小的AgB 4峰。未回收AgB 29和非AgB抗原。由于AgB 29在提取物中浓度很高且显示不太不稳定，提示它可能存在于与携带AgB 4、26和30的分子不同的分子上。
