一种制备UDP-β-L-(U-14C)鼠李糖的改良方法。
A modified procedure for the preparation of UDP-beta-L-(U-14C)rhamnose.
作者信息
Kamsteeg J, van Brederode J, Kuipers-Tierie E, van Nigtevecht G
出版信息
Prep Biochem. 1979;9(4):457-64. doi: 10.1080/00327487908061706.
PMID:41233
Abstract
This paper describes the synthesis of UDP-L-(U-14C)rhamnose from UDP-D-(U-14C)glucose and NADPH using an enzyme preparation of Nicotiana tabacum var. Xanthi. A procedure to separate UDP-l-rhamnose from the other compounds in the reaction mixture is described. Optimal separation was achieved in ethanol 95%-1 M ammonium acetate (pH 3.8) (7:3, v/v) at 30 degrees C.
摘要
本文描述了使用烟草品种黄花烟草的酶制剂，从UDP-D-(U-¹⁴C)葡萄糖和NADPH合成UDP-L-(U-¹⁴C)鼠李糖的过程。文中还描述了一种从反应混合物中的其他化合物中分离UDP-L-鼠李糖的方法。在30℃下，于95%乙醇-1M醋酸铵（pH 3.8）（7:3，v/v）中可实现最佳分离。
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