Bonhomme-Florentin A, Hempel-Zawitkowska J
Ann Nutr Aliment. 1977;31(3):331-48.
The behaviour of rumen ciliates in culture in vitro is studied according to the concentration of substrates and requirements of middle removal. The most favorable concentrations, for a best ciliate's survival is from 0.4 to 0.6 mg/ml of sucrose and 0.05 mg/ml of urea. We observe two periods in the culture: in the first, Entodinium grows essentially, in the second the population of holotrich ciliates increases. If survival is almost the same, the population is most important in culture with sucrose urea than with sucrose.
根据底物浓度和中间产物去除需求,研究了瘤胃纤毛虫在体外培养中的行为。对于纤毛虫的最佳存活而言,最适宜的浓度是蔗糖0.4至0.6毫克/毫升和尿素0.05毫克/毫升。我们在培养过程中观察到两个阶段:第一阶段,内毛虫主要生长;第二阶段,全毛纤毛虫数量增加。如果存活率几乎相同,那么在含有蔗糖和尿素的培养物中,种群数量比仅含蔗糖的培养物更为重要。