Borisova I G, Chepurenko N V, Budnitskaia E V
Biokhimiia. 1977 Nov;42(11):2079-85.
The isoenzyme composition and some properties of lipoxygenase isolated from the seeds and 10-day old sprouts of pea plant were studied. The enzyme activity assay, using gel- and ion-exchange chromatography, disc-electrophoresis in polyacrylamide gel, etc. revealed that the plant contains two lipoxygenase systems of unsaturated long-chain fatty acids oxidation. The existence of four lipoxygenase isoenzymes whose combination determines the type of lipoxygenase-catalyzed reactions of linoleic acid oxidation, has been confirmed.
对从豌豆植株的种子和10日龄豆芽中分离出的脂氧合酶的同工酶组成及某些特性进行了研究。通过酶活性测定、凝胶和离子交换色谱法、聚丙烯酰胺凝胶圆盘电泳等方法发现，该植物含有两个不饱和长链脂肪酸氧化的脂氧合酶系统。已经证实存在四种脂氧合酶同工酶，它们的组合决定了脂氧合酶催化的亚油酸氧化反应的类型。
