番茄斑萎病毒的蛋白质组成
Protein composition of tomato spotted wilt virus.
作者信息
Mohamed N A, Randles J W, Francki R I
出版信息
Virology. 1973 Nov;56(1):12-21. doi: 10.1016/0042-6822(73)90283-3.
Analysis of the protein composition of tomato spotted wilt virus (TSWV), purified by an improved procedure, by polacrylamide gel electrophoresis, revealed three major structural proteins (of MW 84,000, 50,000, and 29,000d) and a minor one of MW 220,000d. The three major proteins constitute about 98% of the total viral protein and all three were shown to be glycoproteins. One of the major proteins (MW 29,000d) and the minor protein were shown to be associated with subviral particles isolated by treatment of virus with the nonionic detergent Nonidet P-40. Only traces of the other two proteins were detected in the subviral particles. Synthesis of virus-induced proteins in TSWV-infected tobacco leaves was studied by labeling infected and healthy tissue with [H]and [C]valine, respectively. The labeled tissues were then fractionated into crude subcellular fractions and protein patterns of healthy and infected tissues were compared by coelectrophoresis on polyacrylamide gels. Only one virus-specific protein (of MW 49,000d) was detected in the virus-enriched fractions; this corresponded with the viral structural protein of MW 50,000d.
采用改进方法纯化番茄斑萎病毒（TSWV），通过聚丙烯酰胺凝胶电泳对其蛋白质组成进行分析，结果显示有三种主要结构蛋白（分子量分别为84,000、50,000和29,000道尔顿）以及一种分子量为220,000道尔顿的次要蛋白。这三种主要蛋白约占病毒总蛋白的98%，且均被证明是糖蛋白。其中一种主要蛋白（分子量29,000道尔顿）和次要蛋白被证明与用非离子去污剂Nonidet P - 40处理病毒后分离得到的亚病毒颗粒有关。在亚病毒颗粒中仅检测到痕量的另外两种蛋白。分别用[H]和[C]缬氨酸标记感染和健康的烟草叶片组织，研究了TSWV感染的烟草叶片中病毒诱导蛋白的合成。然后将标记的组织分级分离成粗亚细胞级分，并通过聚丙烯酰胺凝胶共电泳比较健康组织和感染组织的蛋白质图谱。在富含病毒的级分中仅检测到一种病毒特异性蛋白（分子量49,000道尔顿）；这与分子量50,000道尔顿的病毒结构蛋白相对应。
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