Rogers H J, Thurman P F
J Bacteriol. 1978 Jan;133(1):298-305. doi: 10.1128/jb.133.1.298-305.1978.
The Mg2+ requirement of a morphological mutant of Bacillus subtlis, rodB strain 104 was highly temperature sensitive in the presence of halide or nitrate anions. Likewise the morphological change from rod shapes to spheres was dependent upon temperature, the same anions, and the Mg2+ concentration. The three factors interacted. Other rodB mutants behaved similarly. If the rodB strain 104 in its rod form was treated at high temperatures in the absence of either protein or peptidoglycan synthesis and restored to lower temperatures with the syntheses restarted, a partial temporary change toward cocci occurred. In the absence of halides or in the presence of Cl- but not Br-, the cells increased in volume when they changed from rods to cocci.
枯草芽孢杆菌形态突变体rodB菌株104对镁离子的需求在卤化物或硝酸根阴离子存在时对温度高度敏感。同样，从杆状形态向球状形态的转变取决于温度、相同的阴离子以及镁离子浓度。这三个因素相互作用。其他rodB突变体表现类似。如果处于杆状形态的rodB菌株104在高温下处理，此时蛋白质或肽聚糖合成均不存在，然后恢复到较低温度并重新开始合成，会发生向球菌的部分暂时转变。在没有卤化物的情况下或在有氯离子但没有溴离子存在时，细胞从杆状转变为球菌时体积会增大。
