使用¹⁴C标记麦醇溶蛋白的抗麦醇溶蛋白抗体放射免疫测定法。

Radioimmunoassay for antigliadin-antibodies using 14C-labelled gliadin.

作者信息

Menzel J

出版信息

J Immunol Methods. 1977;18(3-4):257-68. doi: 10.1016/0022-1759(77)90180-6.

Abstract

A sensitive radioimmunoassay for antibodies to gliadin has been developed. Gliadin from wheat gluten was labelled with [1-14C]acetic anhydride to a specific activity of 2.6 X 10(6) dpm/mg. Immunological evidence is presented that the antigen was not essentially altered by the labelling procedure. Experimentally-induced antigliadin antibodies or sera of patients with coeliac disease (CD) were reacted with labelled gliadin and the immune complexes formed precipitated by antiglobulin. Precipitating antibodies were determined by incubating CD sera with labelled gliadin and measuring the radioactivity in precipitates formed without the addition of second antibody. Comparison with other methods for the detection of antigliadin antibodies, including immunoelectrophoresis, immunodiffusion and passive hemagglutination indicated that total and precipitating antibodies were determined only by RIA. The assay also provides information on the immunoglobulin class of antigliadin-antibodies present in sera of patients with coeliac disease.

摘要

已开发出一种检测抗麦醇溶蛋白抗体的灵敏放射免疫测定法。从小麦面筋中提取的麦醇溶蛋白用[1-¹⁴C]乙酸酐标记，比活度为2.6×10⁶ dpm/mg。有免疫学证据表明，标记过程并未使抗原发生本质改变。将实验诱导产生的抗麦醇溶蛋白抗体或乳糜泻（CD）患者的血清与标记的麦醇溶蛋白反应，形成的免疫复合物用抗球蛋白沉淀。通过将CD血清与标记的麦醇溶蛋白孵育，并测量在不添加第二抗体的情况下形成的沉淀物中的放射性，来测定沉淀抗体。与其他检测抗麦醇溶蛋白抗体的方法（包括免疫电泳、免疫扩散和被动血凝反应）进行比较表明，总抗体和沉淀抗体仅通过放射免疫测定法来测定。该测定法还可提供有关乳糜泻患者血清中存在的抗麦醇溶蛋白抗体免疫球蛋白类别的信息。