使用变性条件进行不溶性表皮蛋白的凝胶扩散。
The use of denaturing conditions for gel diffusion of insoluble epidermal proteins.
作者信息
Lee L D, Baden H P
出版信息
J Immunol Methods. 1977;18(3-4):381-5. doi: 10.1016/0022-1759(77)90192-2.
PMID:412900
Abstract
Modifications of the classical technique for double diffusion in agar plates are presented. These procedures allow insoluble proteins to be dissolved in a variety of denaturing solvents and antibody production specificity to be monitored by the appearance of precipitin lines in agarose gels as in the conventional Ouchterlony method. Results obtained using the insoluble proteins of bovine epidermis are summarized.
摘要
本文介绍了琼脂平板双扩散经典技术的改良方法。这些方法能使不溶性蛋白质溶解于多种变性溶剂中，并像传统的欧氏免疫扩散法一样，通过琼脂糖凝胶中沉淀线的出现来监测抗体产生的特异性。文中总结了使用牛表皮不溶性蛋白质所获得的结果。
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