钙与乌贼巨大轴突膜的关联：超微结构与微探针分析
Association of calcium with membranes of squid giant axon: ultrastructure and microprobe analysis.
作者信息
Oschman J L, Hall T A, Peters P D, Wall B J
出版信息
J Cell Biol. 1974 Apr;61(1):156-65. doi: 10.1083/jcb.61.1.156.
Abstract
Giant axons from the squid, Loligo pealei, were fixed in glutaraldehyde and postfixed in osmium tetroxide. Calcium chloride (5 mM/liter) was added to all aqueous solutions used for tissue processing. Electron-opaque deposits were found along the axonal plasma membranes, within mitochondria, and along the basal plasma membranes of Schwann cells. X-ray microprobe analysis (EMMA-4) yielded signals for calcium and phosphorus when deposits were probed, whereas these elements were not detected in the axoplasm.
摘要
来自枪乌贼（Loligo pealei）的巨大轴突用戊二醛固定，并用四氧化锇进行后固定。在用于组织处理的所有水溶液中添加了氯化钙（5 mM/升）。在轴突质膜、线粒体内以及雪旺细胞的基底质膜上发现了电子不透明沉积物。当对沉积物进行探测时，X射线微探针分析（EMMA-4）产生了钙和磷的信号，而在轴浆中未检测到这些元素。
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