Lüth H J, Seidel J, Schober W
Acta Histochem. 1977;60(1):91-102.
After selective application of horseradish peroxidase (HRP) into the dorsal part of the lateral geniculate nucleus (LGN) of the albino rat, cells were labelled through the retrograde transport of the enzyme in the following nuclei of the brain stem: 1. Ncl. raphes linearis, 2. Nel, raphes centralis, 3. Ncl. raphes dorsalis, 4. Ncl. ventralis rostralis lemnisci lateralis, 5. Ncl. coeruleus. Using the glyoxylic acid method to demonstrate biogenic amines we could find serotonine in following areas of the brain stem: 1. region B 7 (Ncl. raphes dorsalis) positive cells; 2. region B 8 (Ncl. raphes centralis) mainly positive fibres; 3. region B 9 (dorsal of the Lemniscus medialis) a few positive cells; 4. mesencephalic reticular formation (Area cuneiformis) network-like fibre structures; 5. dorsal LGN, in the lateral part mildly fluorescing fibres, the remaining LGN showing diffuse fluorescence, 6. Substantia nigra, fluorescing cells. The monosynaptic connections between the mesencephalic raphe nuclei and the dorsal part of the LGN, demonstrated by means of the HRP-method, are characterized as being serotoninergic with the help of fluorescence histochemistry. The character of the transmitter of these connections is compared with results of biochemical and pharmacological investigations.
将辣根过氧化物酶(HRP)选择性注入白化大鼠外侧膝状体核(LGN)的背侧部分后,通过该酶在脑干以下核团中的逆行运输对细胞进行标记:1. 中缝线性核;2. 中缝中央核;3. 中缝背核;4. 外侧丘系腹侧吻核;5. 蓝斑核。使用乙醛酸法显示生物胺,我们可以在脑干的以下区域发现5-羟色胺:1. B7区(中缝背核)阳性细胞;2. B8区(中缝中央核)主要为阳性纤维;3. B9区(内侧丘系背侧)少量阳性细胞;4. 中脑网状结构(楔形区)呈网络状的纤维结构;5. 外侧膝状体核背侧,外侧部分有轻度荧光纤维,其余外侧膝状体核呈弥漫性荧光;6. 黑质,有荧光细胞。通过HRP法证实的中脑缝际核与外侧膝状体核背侧之间的单突触联系,借助荧光组织化学被表征为5-羟色胺能。将这些联系的递质特性与生化和药理学研究结果进行了比较。