Berg W, Hillvärn B, Arwin H, Stenberg M, Lundström I
Thromb Haemost. 1979 Oct 31;42(3):972-82.
We have shown that the isoelectric point of thrombin is high and that thrombin is a cation at the pH of blood. On the other hand, prothrombin has a low isoelectric point, being more anionic at the pH of blood. It was also found that thrombin adsorbs readily to surfaces, especially negatively charged surfaces, like behenic acid surfaces at pH 8.2. Furthermore, thrombin adsorbed onto behenic acid was active in the sense that it coagulated fibrinogen. The significance of the electric charge of the thrombin molecule in the mechanism of atherosclerosis is discussed.
我们已经表明，凝血酶的等电点较高，在血液pH值下是阳离子。另一方面，凝血酶原的等电点较低，在血液pH值下更具阴离子性。还发现凝血酶很容易吸附到表面，特别是带负电荷的表面，如pH 8.2的山嵛酸表面。此外，吸附在山嵛酸上的凝血酶在使纤维蛋白原凝固的意义上是有活性的。讨论了凝血酶分子电荷在动脉粥样硬化机制中的意义。
