对NZB-NZW F1小鼠自发性淋巴瘤细胞上独特型决定簇的保护性和细胞免疫反应。
Protective and cellular immune responses to idiotypic determinants on cells from a spontaneous lymphoma of NZB-NZW F1 mice.
作者信息
Sugai S, Palmer D W, Talal N, Witz I P
出版信息
J Exp Med. 1974 Dec 1;140(6):1547-58. doi: 10.1084/jem.140.6.1547.
A spontaneous lymphoma (141) producing monoclonal IgM is established in NZB/NZW F(1) (B/W) mice who spontaneously develop an autoimmune disease. Idiotypic determinants of 141 IgM are present on the lymphoma cell surface as shown by indirect immunofluorescence and specific cytotoxicity with rabbit anti-idiotypic antiserum. Fluorescence and cytotoxicity are inhibited by 141 IgM but not by 104E IgM, a monoclonal IgM produced by a BALB/c plasmacytoma. Immunization of B/W mice with 141 IgM before transplantation of lymphoma 141 confers protective immunity. No such protection occurs after immunization with 104E IgM or other unrelated proteins. Protected mice contain spleen cells cytotoxic for 141 lymphoma cells. This cytotoxicity is blocked by incubation of spleen cells with 141 IgM but not with 104E IgM. Moreover, splenic lymphocytes from protected mice are stimulated to synthesize DNA by 141 IgM but not by 104E IgM. These results suggest that specific cellular immune responses to idiotypic determinants may participate in the observed protection against challenge with the corresponding B-cell tumor.
在自发患上自身免疫性疾病的NZB/NZW F(1)（B/W）小鼠中建立了一种产生单克隆IgM的自发性淋巴瘤（141）。如间接免疫荧光和兔抗独特型抗血清的特异性细胞毒性所示，141 IgM的独特型决定簇存在于淋巴瘤细胞表面。荧光和细胞毒性被141 IgM抑制，但不被BALB/c浆细胞瘤产生的单克隆IgM 104E IgM抑制。在移植淋巴瘤141之前用141 IgM免疫B/W小鼠可赋予保护性免疫。用104E IgM或其他无关蛋白免疫后不会产生这种保护作用。受保护的小鼠含有对141淋巴瘤细胞具有细胞毒性的脾细胞。这种细胞毒性可通过将脾细胞与141 IgM孵育而被阻断，但与104E IgM孵育则不会。此外，来自受保护小鼠的脾淋巴细胞被141 IgM刺激合成DNA，但不被104E IgM刺激。这些结果表明，对独特型决定簇的特异性细胞免疫反应可能参与了观察到的针对相应B细胞肿瘤攻击的保护作用。
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