与特定哺乳动物基因对应的固相结合DNA序列的酶促合成。
Enzymatic synthesis of solid phase-bound DNA sequences corresponding to specific mammalian genes.
作者信息
Venetianer P, Leder P
出版信息
Proc Natl Acad Sci U S A. 1974 Oct;71(10):3892-5. doi: 10.1073/pnas.71.10.3892.
Abstract
With oligo(dT)-cellulose as primer, RNA-dependent DNA polymerase catalyzes the synthesis of cellulose-bound DNA that is complementary to mouse globin mRNA. The resulting cellulose-bound or solid phase complementary DNA hybridizes specifically with globin mRNA and permits the recovery of intact globin mRNA. This simple technique for the synthesis of solid phase-bound complementary DNA provides an additional and convenient method for the purification of specific genetic sequences.
摘要
以寡聚（dT）-纤维素为引物，依赖RNA的DNA聚合酶催化合成与小鼠珠蛋白mRNA互补的纤维素结合DNA。所产生的纤维素结合或固相互补DNA与珠蛋白mRNA特异性杂交，并能回收完整的珠蛋白mRNA。这种合成固相结合互补DNA的简单技术为特定基因序列的纯化提供了一种额外且便捷的方法。
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