Tedesco T A, Diamond R, Orkwiszewski K G, Boedecker H J, Croce C M
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3483-6. doi: 10.1073/pnas.71.9.3483.
Mouse-human hybrid clones were tested for the presence of human hexose-1-phosphate uridylyl-transferase (EC 2.7.7.12;UDPglucose:alpha-D-galactose-1-phosphate uridylyltransferase). Two criteria, starch gel electrophoresis and double-immunodiffusion against a human transferase-specific antibody, were used to identify human enzyme in the hybrid clones. Seventeen of 33 hybrid clones analyzed were found to contain human transferase by both criteria. Karyological analysis of the hybrid clones showed concordant segregation of human transferase with human chromosome 3. Human galactokinase was asyntenic with human transferase. We thus assign this gene to human chromosome 3.
对小鼠 - 人杂交克隆进行检测，以确定是否存在人己糖 - 1 - 磷酸尿苷酰转移酶（EC 2.7.7.12；UDP葡萄糖：α - D - 半乳糖 - 1 - 磷酸尿苷酰转移酶）。使用淀粉凝胶电泳和针对人转移酶特异性抗体的双向免疫扩散这两个标准，来鉴定杂交克隆中的人酶。通过这两个标准分析的33个杂交克隆中，有17个被发现含有人转移酶。对杂交克隆的染色体分析表明，人转移酶与人3号染色体呈一致分离。人半乳糖激酶与人转移酶不同源。因此，我们将该基因定位到人3号染色体上。
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