Rautenshteĭn Ia I, Zhunaeva V V, Solov'eva N Ia
Mikrobiologiia. 1977 Nov-Dec;46(6):1095-1102.
A polylysogenic culture of Actinomyces griseus 15 producing the antibiotic cormogrisin is sensitive to actinophages, denoted as types 15 I, 15 II and 15 III, which are the virulent mutants of its temperate phages. In certain cases, the variants of Act. griseus 15, obtained by selection and resistant to the phages types 15 I and 15 II, can undergo lysis-from-without by these phages. A study of the mechanisms involved in this phenomenon has shown that the lytic activity is entirely eliminated if the phages have been a titre of 10(9) and higher. The lytic activity is entirely eliminated if the phages have been treated with a specific serum or inactivated by UV. The variants resistant to phage 15 I can adsorb this phage and undergo lysis-from-without at a high multiplicity of infection if only the age of the variant does not exceed 13 hours. The variants resistant to phages 15 I and 15 II can undergo lysis-from-within under the action of phage 15 III. The variants resistant to this phage have acquired this resistance as a result of lysogenization. They have entirely retained the ability to adsorb phage 15 I, but their ability to absorb phage 15 III is much lower. Therefore, the rate of mycelium growth is only slightly inhibited, but the lysis of the culture is not observed, under the action of phage 15 III having titres of 10(9) and 10(10).
产生抗生素灰霉素的灰色链霉菌15的多溶原培养物对肌动噬菌体敏感，这些噬菌体被标记为15 I型、15 II型和15 III型，它们是其温和噬菌体的烈性突变体。在某些情况下，通过筛选获得的对15 I型和15 II型噬菌体具有抗性的灰色链霉菌15变体，可被这些噬菌体从外部进行裂解。对这一现象所涉及机制的研究表明，如果噬菌体的效价达到10(9)及更高，则裂解活性会完全消除。如果噬菌体用特异性血清处理或经紫外线灭活，裂解活性也会完全消除。对15 I型噬菌体具有抗性的变体能够吸附这种噬菌体，并且如果变体的菌龄不超过13小时，在高感染复数时会从外部进行裂解。对15 I型和15 II型噬菌体具有抗性的变体在15 III型噬菌体的作用下会从内部进行裂解。对这种噬菌体具有抗性的变体是通过溶原化获得这种抗性的。它们完全保留了吸附15 I型噬菌体的能力，但吸附15 III型噬菌体的能力要低得多。因此，在效价为10(9)和10(10)的15 III型噬菌体作用下，菌丝体生长速率仅受到轻微抑制，但未观察到培养物的裂解现象。
