用于抗酸菌染色的纸条法与齐-尼氏法的比较。
Comparison of strip and Ziehl-Neelsen methods for staining acid-fast bacteria.
作者信息
Varughese P, Helbecque D M, McRae K B, Eidus L
出版信息
Bull World Health Organ. 1974;51(1):83-91.
The efficiency of the Ziehl-Neelsen method for staining acid-fast bacteria was compared with that of the strip-staining procedure in which one kind of impregnated strip is used to stain the bacteria and another kind for simultaneous decolorization and counter-staining of the smear. The methods were evaluated in 1 136 duplicate smears prepared from digested sputum and 307 pairs of direct smears. The efficiency of the strip method was comparable to that of the Ziehl-Neelsen method with digested sputum; with direct smears, however, it generally depended on the quality of the smear. With thick, uneven smears, lower bacterial counts were obtained by strip staining. On the basis of this trial, the authors suggest improvement of the strip method.
将萋-尼氏法（Ziehl-Neelsen method）用于抗酸菌染色的效率，与条带染色法进行了比较。条带染色法使用一种浸渍条带对细菌进行染色，另一种用于对涂片同时进行脱色和复染。在1136份由消化痰液制备的重复涂片以及307对直接涂片上对这些方法进行了评估。条带法的效率与萋-尼氏法用于消化痰液染色时相当；然而，对于直接涂片，其效率通常取决于涂片的质量。对于厚且不均匀的涂片，条带染色获得的细菌计数较低。基于该试验，作者建议对条带法进行改进。
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