Jones D G, Kay A B
Clin Exp Immunol. 1974 Feb;16(2):213-22.
Guinea-pig skin fragments were passively sensitized for the antigen-induced release of histamine, SRS-A and ECF-A. Skin histamine was chemically identified by fluorimetry; SRS-A gave a characteristic dose-dependent contraction of the guinea-pig ileum; and ECF-A selectively attracted eosinophils. The antibody mediating the release of these agents was shown to be IgG. Following antigen challenge different time courses of release were demonstrable for histamine, SRS-A and ECF-A. Skin SRS-A was resistant to treatment with the enzyme pronase and skin ECF-A had an estimated molecular size of between 500 and 1000. They were therefore comparable to similar agents released from the lung. Thus SRS-A and ECF-A join histamine as chemical mediators in cutaneous anaphylaxis.
豚鼠皮肤碎片被被动致敏以用于抗原诱导的组胺、慢反应物质A（SRS-A）和嗜酸性粒细胞趋化因子A（ECF-A）的释放。皮肤组胺通过荧光法进行化学鉴定；SRS-A使豚鼠回肠产生特征性的剂量依赖性收缩；而ECF-A选择性地吸引嗜酸性粒细胞。介导这些介质释放的抗体被证明是IgG。抗原激发后，组胺、SRS-A和ECF-A呈现出不同的释放时间进程。皮肤SRS-A对链霉蛋白酶处理具有抗性，皮肤ECF-A的估计分子大小在500至1000之间。因此，它们与从肺释放的类似介质相当。这样，SRS-A和ECF-A与组胺一起成为皮肤过敏反应中的化学介质。
