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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Harwood J L, Sodja A, Stumpf P K

Biochem J. 1972 Dec;130(4):1013-8. doi: 10.1042/bj1301013.

An avocado supernatant fraction converted fatty acids of medium chain length (C(8)-C(12)) into a polar product. 2. The product was identified as the beta-hydroxy derivative of the substrate by g.l.c. and t.l.c. analysis. 3. For hydroxylation of the fatty acids, CoA, ATP and molecular oxygen were required. Acyl carrier protein gave some stimulation. The reaction took place with oxygen alone if acyl-CoA was the substrate. 4. Hydroxylation was maximal with decanoic acid but dodecanoic acid and octanoic acid were also very active. Acids of shorter or longer chain lengths were not hydroxylated. 5. NAD(+) concentration caused complete inhibition at 0.5mm and may be an important control mechanism for the reaction in vivo. 6. The reaction was inhibited by iodoacetamide and by bipyridyl and carbon monoxide, indicating involvement of thiol and heavy metal groups.

鳄梨上清液组分将中链长度（C(8)-C(12)）的脂肪酸转化为一种极性产物。2. 通过气相色谱（g.l.c.）和薄层层析（t.l.c.）分析，该产物被鉴定为底物的β-羟基衍生物。3. 脂肪酸羟基化需要辅酶A（CoA）、三磷酸腺苷（ATP）和分子氧。酰基载体蛋白有一定的促进作用。如果酰基辅酶A作为底物，仅在有氧气的情况下反应发生。4. 癸酸的羟基化作用最强，但十二烷酸和辛酸也非常活跃。链长较短或较长的酸不会发生羟基化。5. 烟酰胺腺嘌呤二核苷酸（NAD(+)）浓度在0.5毫摩尔时会导致完全抑制，这可能是该反应在体内的一种重要调控机制。6. 该反应受到碘乙酰胺、联吡啶和一氧化碳的抑制，表明硫醇和重金属基团参与其中。

Harwood J L, Sodja A, Stumpf P K

Biochem J. 1972 Dec;130(4):1013-8. doi: 10.1042/bj1301013.

An avocado supernatant fraction converted fatty acids of medium chain length (C(8)-C(12)) into a polar product. 2. The product was identified as the beta-hydroxy derivative of the substrate by g.l.c. and t.l.c. analysis. 3. For hydroxylation of the fatty acids, CoA, ATP and molecular oxygen were required. Acyl carrier protein gave some stimulation. The reaction took place with oxygen alone if acyl-CoA was the substrate. 4. Hydroxylation was maximal with decanoic acid but dodecanoic acid and octanoic acid were also very active. Acids of shorter or longer chain lengths were not hydroxylated. 5. NAD(+) concentration caused complete inhibition at 0.5mm and may be an important control mechanism for the reaction in vivo. 6. The reaction was inhibited by iodoacetamide and by bipyridyl and carbon monoxide, indicating involvement of thiol and heavy metal groups.

鳄梨上清液组分将中链长度（C(8)-C(12)）的脂肪酸转化为一种极性产物。2. 通过气相色谱（g.l.c.）和薄层层析（t.l.c.）分析，该产物被鉴定为底物的β-羟基衍生物。3. 脂肪酸羟基化需要辅酶A（CoA）、三磷酸腺苷（ATP）和分子氧。酰基载体蛋白有一定的促进作用。如果酰基辅酶A作为底物，仅在有氧气的情况下反应发生。4. 癸酸的羟基化作用最强，但十二烷酸和辛酸也非常活跃。链长较短或较长的酸不会发生羟基化。5. 烟酰胺腺嘌呤二核苷酸（NAD(+)）浓度在0.5毫摩尔时会导致完全抑制，这可能是该反应在体内的一种重要调控机制。6. 该反应受到碘乙酰胺、联吡啶和一氧化碳的抑制，表明硫醇和重金属基团参与其中。