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蓝藻中吡啶核苷酸依赖性葡萄糖脱氢酶活性

Pyridine nucleotide-dependent glucose dehydrogenase activity in blue-green algae.

作者信息

Pulich W M, Van Baalen C

出版信息

J Bacteriol. 1973 Apr;114(1):28-33. doi: 10.1128/jb.114.1.28-33.1973.

Abstract

Pyridine nucleotide-dependent glucose dehydrogenase activity (GPND) is described for the first time in cell-free extracts of certain blue-green algae. When glucose is added to these crude cell extracts, nicotinamide adenine dinucleotide phosphate is reduced at twice the rate as nicotinamide adenine dinucleotide; but evidence suggests that this activity is due to a single enzyme. The distribution and level of GPND in selected blue-green algae correlates with the heterotrophic potential of each species. In all blue-green algae where GPND was detected, O(2) uptake coupled to the GPND reaction was also observed. Both GPND and O(2) uptake apparently occur in the soluble fraction of the cell. An essential role for GPND in the heterotrophic metabolism of blue-green algae is postulated.

摘要

首次在某些蓝藻的无细胞提取物中描述了吡啶核苷酸依赖性葡萄糖脱氢酶活性(GPND)。当向这些粗细胞提取物中添加葡萄糖时,烟酰胺腺嘌呤二核苷酸磷酸的还原速率是烟酰胺腺嘌呤二核苷酸的两倍;但有证据表明这种活性归因于单一酶。所选蓝藻中GPND的分布和水平与每个物种的异养潜力相关。在所有检测到GPND的蓝藻中,还观察到与GPND反应偶联的氧气摄取。GPND和氧气摄取显然都发生在细胞的可溶部分。推测GPND在蓝藻的异养代谢中起重要作用。

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本文引用的文献

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HETEROTROPHY AND NITROGEN FIXATION IN CHLOROGLOEA FRITSCHII.绿胶藻的异养与固氮作用
J Gen Microbiol. 1965 Apr;39:11-20. doi: 10.1099/00221287-39-1-11.
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The endogenous metabolism of microorganisms.微生物的内源性代谢
Annu Rev Microbiol. 1962;16:241-64. doi: 10.1146/annurev.mi.16.100162.001325.
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Heterotrophic growth of blue-gren algae in dim light.蓝绿藻在弱光下的异养生长。
J Bacteriol. 1971 Mar;105(3):685-9. doi: 10.1128/jb.105.3.685-689.1971.

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