Steensma H Y, Sondermeijer P J
Antonie Van Leeuwenhoek. 1977;43(3-4):305-16. doi: 10.1007/BF02313757.
The defective phages of Bacillus subtilis cannot be counted by plating as they do not form plaques. In addition, counting under the electron microscope with latex spheres as an internal standard is not possible. The reliability of a method using Escherichia coli phage T4 as a substitute for the latex spheres has been tested and the results compared with those of other methods. Using this method, we determined the burst sizes of the defective phages PBS X, PBS Y and PBS Z under various conditions.
枯草芽孢杆菌的缺陷噬菌体无法通过平板计数，因为它们不会形成噬菌斑。此外，以乳胶球作为内标在电子显微镜下计数也是不可能的。已经测试了一种使用大肠杆菌噬菌体T4替代乳胶球的方法的可靠性，并将结果与其他方法的结果进行了比较。使用这种方法，我们测定了缺陷噬菌体PBS X、PBS Y和PBS Z在各种条件下的裂解量。
