肺炎球菌DNA诱导转化中的整合效率。I. 固体培养基中的转化方法及其在分离转化缺陷型和重组修饰突变体中的应用。
Integration efficiency in DNA-induced transformation of Pneumococcus. I. A method of transformation in solid medium and its use for isolation of transformation-deficient and recombination-modified mutants.
作者信息
Tiraby G, Claverys J P, Sicard M A
出版信息
Genetics. 1973 Sep;75(1):23-33. doi: 10.1093/genetics/75.1.23.
A method of transformation on solid medium especially adapted for pneumococcus has been developed. Under specific conditions, all colonies that are allowed to grow in the presence of transforming DNA for six hours give rise to transformed bacteria. Combined with replica plating this technique has been used to isolate mutants modified with regard to recombination. Most of the mutants found are transformation-defective and show a large diversity in their response to ultraviolet light. Some of these mutants have lost their ability to take up transforming DNA. One shows a reduced yield of transformants for a given quantity of DNA taken up. Mutants that manifest altered behavior with regard to marker efficiencies have also been isolated. One of these exhibits a decrease in the transformation efficiency of only the high efficiency markers and two mutants show a decrease in the transformation efficiency of the low efficiency markers.
已开发出一种特别适用于肺炎球菌的固体培养基转化方法。在特定条件下，所有在转化DNA存在下生长6小时的菌落都会产生转化细菌。结合影印平板培养法，该技术已被用于分离在重组方面发生改变的突变体。发现的大多数突变体是转化缺陷型的，并且在对紫外线的反应上表现出很大的差异。其中一些突变体失去了摄取转化DNA的能力。有一个突变体在摄取给定数量的DNA时，转化子产量降低。还分离出了在标记效率方面表现出行为改变的突变体。其中一个仅表现出高效标记的转化效率降低，还有两个突变体表现出低效标记的转化效率降低。
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