Vorhaben J E, Wong L, Campbell J W
Biochem J. 1973 Dec;135(4):893-6. doi: 10.1042/bj1350893.
The use of phosphoenolpyruvate plus pyruvate kinase as an ATP-generating system in the assay for glutamine synthetase activity via the formation of gamma-glutamylhydroxamate from glutamate and hydroxylamine with crude tissue preparations is shown to give values far in excess of the true glutamine synthetase activity of the tissue. This is due to the generation of pyruvate, which reacts with hydroxylamine to give a compound that is chromogenic with the ferric chloride reagent used for measuring gamma-glutamylhydroxamate.
在通过用粗制组织制剂使谷氨酸和羟胺形成γ-谷氨酰羟肟酸来测定谷氨酰胺合成酶活性的实验中，使用磷酸烯醇丙酮酸加丙酮酸激酶作为ATP生成系统，结果显示所得值远远超过该组织真正的谷氨酰胺合成酶活性。这是由于丙酮酸的生成，丙酮酸与羟胺反应生成一种化合物，该化合物与用于测定γ-谷氨酰羟肟酸的氯化铁试剂会发生显色反应。
