谷氨酰胺合成酶活性测定
Assay for glutamine synthetase activity.
作者信息
Vorhaben J E, Wong L, Campbell J W
出版信息
Biochem J. 1973 Dec;135(4):893-6. doi: 10.1042/bj1350893.
Abstract
The use of phosphoenolpyruvate plus pyruvate kinase as an ATP-generating system in the assay for glutamine synthetase activity via the formation of gamma-glutamylhydroxamate from glutamate and hydroxylamine with crude tissue preparations is shown to give values far in excess of the true glutamine synthetase activity of the tissue. This is due to the generation of pyruvate, which reacts with hydroxylamine to give a compound that is chromogenic with the ferric chloride reagent used for measuring gamma-glutamylhydroxamate.
摘要
在通过用粗制组织制剂使谷氨酸和羟胺形成γ-谷氨酰羟肟酸来测定谷氨酰胺合成酶活性的实验中，使用磷酸烯醇丙酮酸加丙酮酸激酶作为ATP生成系统，结果显示所得值远远超过该组织真正的谷氨酰胺合成酶活性。这是由于丙酮酸的生成，丙酮酸与羟胺反应生成一种化合物，该化合物与用于测定γ-谷氨酰羟肟酸的氯化铁试剂会发生显色反应。
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Assay for glutamine synthetase activity.谷氨酰胺合成酶活性测定
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本文引用的文献
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Studies on the enzymic synthesis of glutamine.谷氨酰胺的酶促合成研究。
Biochem J. 1951 Jun;49(1):106-12. doi: 10.1042/bj0490106.
2
Studies on the mechanism of glutamine synthesis; isolation and properties of the enzyme from sheep brain.谷氨酰胺合成机制的研究；绵羊脑组织中该酶的分离与特性
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Glutamine synthetase. Determination of its distribution in brain during development.谷氨酰胺合成酶。其在大脑发育过程中的分布测定。
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Glutamine synthetase activity in tissues of lower vertebrates.
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Glutamine synthetase. A mitochondrial enzyme in uricotelic species.谷氨酰胺合成酶。尿酸排泄型物种中的一种线粒体酶。
J Biol Chem. 1972 May 10;247(9):2763-7.
6
The distribution between gamma-glutamylhydrozamate synthetase and L-glutamine-hydroxylamine glutamyltransferase activities in rat tissues. Studies in vitro.大鼠组织中γ-谷氨酰异羟肟酸合成酶与L-谷氨酰胺-羟胺谷氨酰转移酶活性的分布。体外研究。
Biochem J. 1973 May;133(1):49-57. doi: 10.1042/bj1330049.
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