Burgen A S, Hiley C R, Young J M
Br J Pharmacol. 1974 Jan;50(1):145-51. doi: 10.1111/j.1476-5381.1974.tb09602.x.
1 The synthesis of tritium labelled propylbenzilylcholine mustard ([(3)H]-PrBCM; N-2'-chloroethyl-N-[2'', 3''-(3)H(2)] propyl-2-aminoethyl benzilate) is described.2 The uptake by muscle strips was measured and shown to be considerably increased by previous immersion of the muscle in distilled water.3 A considerable part of the uptake is inhibited selectively by atropine, but not by nicotinic antagonists. A number of muscarinic agonists also inhibit uptake and their apparent affinity constants have been determined.4 The uptake by atropine-sensitive sites is temperature-insensitive, whereas the other sites are temperature-sensitive. Recovery is highly temperature-sensitive and there is good agreement between recovery of sensitivity to agonists and loss of radioactivity from the muscle.
描述了氚标记的丙基苯甲酰胆碱氮芥（[(3)H]-PrBCM；N-2'-氯乙基-N-[2'', 3''-(3)H(2)]丙基-2-氨基乙基苯甲酸酯）的合成。
测量了肌肉条带的摄取情况，结果表明，之前将肌肉浸入蒸馏水中会使其摄取量显著增加。
摄取的很大一部分被阿托品选择性抑制，但不受烟碱拮抗剂抑制。一些毒蕈碱激动剂也抑制摄取，并已测定了它们的表观亲和常数。
对阿托品敏感位点的摄取对温度不敏感，而其他位点对温度敏感。恢复对温度高度敏感，并且对激动剂的敏感性恢复与肌肉中放射性的丧失之间有很好的一致性。
