Rostenberg I, Guízar-Vázquez J, Suárez P, Rico R, Nungaray L, Domínguez C
J Natl Cancer Inst. 1978 Jan;60(1):83-7. doi: 10.1093/jnci/60.1.83.
Concanavalin A and wheat germ agglutinin, lectins that interact with serum glycoprotein in a manner similar to the antigen--antibody reaction, were used as "antibodies" in a single radial immunodiffusion technique to test a coded serum panel (from the National Cancer Institute, Bethesda, Md., and the Mayo Clinic, Rochester, Minn.) containing a) 99 serum samples from patients with different types of malignant neoplasms of the gastrointestinal tract, prostate gland, and lung, b) 50 samples from patients with benign diseases of the same organs as those affected in the cancer patients, and c) 50 samples from apparently healthy smokers. The resulting precipitation rings were not correlated to serum protein concentration, and the differences (demonstrated by Student's t-test and with a generalization of the one-sided two-sample Kolmogorov-Smirnov statistic for evaluating diagnostic tests) established that serum glycoproteins are glycosylated differently in cancer patients than in people without cancer.
伴刀豆球蛋白A和麦胚凝集素，这两种凝集素以类似于抗原 - 抗体反应的方式与血清糖蛋白相互作用，在单向免疫扩散技术中用作“抗体”，以检测一组编码血清样本（来自马里兰州贝塞斯达的国家癌症研究所和明尼苏达州罗切斯特的梅奥诊所），其中包括：a）99份来自患有胃肠道、前列腺和肺部不同类型恶性肿瘤患者的血清样本，b）50份来自与癌症患者受影响器官相同的良性疾病患者的样本，以及c）50份来自明显健康的吸烟者的样本。所得沉淀环与血清蛋白浓度无关，并且差异（通过学生t检验以及用于评估诊断测试的单侧双样本柯尔莫哥洛夫 - 斯米尔诺夫统计量的推广来证明）表明癌症患者血清糖蛋白的糖基化与无癌症人群不同。
