豚鼠棕色脂肪组织中长链酰基辅酶A水解酶和酰基-L-肉碱水解酶的细胞内定位
Intracellular localization of long-chain acyl-coenzyme A hydrolase and acyl-L-carnitine hydrolase in brown adipose tissue from guinea pigs.
作者信息
Berge R K, Slinde E, Farstad M
出版信息
Biochem J. 1979 Aug 15;182(2):347-51. doi: 10.1042/bj1820347.
The activities of long-chain acyl-CoA hydrolase (palmitoyl-CoA hydrolase, EC 3.1.2.2) and long-chain acyl-L-carnitine hydrolase, EC 3.1.1.28) in brown adipose tissue from cold-exposed and control guinea pigs were studied. Mitochondria from cold-exposed animals hydrolysed 21 nmol of palmitoyl-CoA/min per mg of protein and 1.3 nmol of palmitoyl-L-carnitine/min per mg of protein, and the specific activities were respectively 2 and 5 times as high in cold-exposed as in control animals. The subcellular-localization studies showed that both the long-chain acyl-CoA hydrolase and long-chain acyl-L-carnitine hydrolase were localized in the mitochondria. A location also in the soluble fraction cannot be excluded. The long-chain acyl-CoA hydrolase activity was doubled when the mitochondria were disrupted; this indicates that the enzyme is localized in the matrix compartment.
研究了冷暴露豚鼠和对照豚鼠棕色脂肪组织中长链酰基辅酶A水解酶（棕榈酰辅酶A水解酶，EC 3.1.2.2）和长链酰基-L-肉碱水解酶（EC 3.1.1.28）的活性。冷暴露动物的线粒体每毫克蛋白质每分钟水解21 nmol棕榈酰辅酶A和每毫克蛋白质每分钟水解1.3 nmol棕榈酰-L-肉碱，冷暴露动物的比活性分别是对照动物的2倍和5倍。亚细胞定位研究表明，长链酰基辅酶A水解酶和长链酰基-L-肉碱水解酶都定位于线粒体中。也不能排除其在可溶部分中的定位。当线粒体被破坏时，长链酰基辅酶A水解酶活性加倍；这表明该酶定位于线粒体基质区室。
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