心脏中的腺苷酸代谢。兔心脏腺苷酸脱氨酶的调节特性。
Adenylate metabolism in the heart. Regulatory properties of rabbit cardiac adenylate deaminase.
作者信息
Barsacchi R, Ranieri-Raggi M, Bergamini C, Raggi A
出版信息
Biochem J. 1979 Aug 15;182(2):361-6. doi: 10.1042/bj1820361.
Abstract
The kinetic properties of a 300-fold purified cardiac AMP deaminase were studied and compared with those of the corresponding enzyme from skeletal muscle. The heart enzyme is activated by ATP and less efficiently by ADP, and is inhibited by Pi, phosphocreatine and GTP. ATP, even at micromolar concentrations, is able to abolish the effects of the inhibitors. The affinity of the enzyme for AMP is low in the absence of activators (Km 3.1 mM), but, in the presence of ATP, becomes as high as that of skeletal-muscle AMP deaminase (Km 0.4 mM). The maximal activation by ATP is observed at alkaline pH (pH 7.5-8.0). Under the same conditions ATP is maximally inhibitory for skeletal-muscle enzyme. These results suggest that AMP deaminase in the heart is always in the activated state, whereas in skeletal muscle the enzyme is active only during exhaustive contractions.
摘要
对纯化了300倍的心脏AMP脱氨酶的动力学特性进行了研究，并与骨骼肌中相应的酶进行了比较。心脏中的这种酶被ATP激活，被ADP激活的效率较低，且受到无机磷酸盐、磷酸肌酸和GTP的抑制。即使在微摩尔浓度下，ATP也能够消除抑制剂的作用。在没有激活剂的情况下，该酶对AMP的亲和力较低（Km为3.1 mM），但在ATP存在时，其亲和力变得与骨骼肌AMP脱氨酶一样高（Km为0.4 mM）。在碱性pH值（pH 7.5 - 8.0）下观察到ATP的最大激活作用。在相同条件下，ATP对骨骼肌酶具有最大抑制作用。这些结果表明，心脏中的AMP脱氨酶始终处于激活状态，而在骨骼肌中，该酶仅在剧烈收缩期间才具有活性。
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本文引用的文献
1
The deaminases of adenosine and adenylic acid in blood and tissues.血液和组织中腺苷及腺苷酸的脱氨酶
Biochem J. 1939 Apr;33(4):479-92. doi: 10.1042/bj0330479.
2
AMP and adenosine aminohydrolases in rat tissues.大鼠组织中的AMP及腺苷氨基水解酶
Acta Biochim Pol. 1962;9:83-93.
3
Cardiac nucleotides in hypoxia: possible role in regulation of coronary blood flow.缺氧时的心脏核苷酸：在冠状动脉血流调节中的可能作用。
Am J Physiol. 1963 Feb;204:317-22. doi: 10.1152/ajplegacy.1963.204.2.317.
4
Adenylate deaminase. 3. Regulation of deamination pathways in extracts of rat heart and lung.腺苷酸脱氨酶。3. 大鼠心脏和肺提取物中脱氨途径的调节。
J Biol Chem. 1967 Nov 25;242(22):5281-8.
5
An improved purification, crystallization, and some properties of rabbit muscle 5'-adenylic acid deaminase.兔肌肉5'-腺苷酸脱氨酶的改进纯化、结晶及某些性质
J Biol Chem. 1967 May 25;242(10):2502-6.
6
Univalent cations as allosteric activators of muscle adenosine 5'-phosphate deaminase.单价阳离子作为肌肉腺苷5'-磷酸脱氨酶的变构激活剂。
J Biol Chem. 1967 Apr 25;242(8):1980-1.
7
8
Release of adenosine from anoxic hearts. Relationship to coronary flow.缺氧心脏中腺苷的释放。与冠状动脉血流的关系。
Circ Res. 1966 Aug;19(2):420-5. doi: 10.1161/01.res.19.2.420.
9
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