[嗜酸热硫化叶菌的5'-甲硫腺苷磷酸化酶。1. 纯化及部分特性鉴定]
[5'-methylthioadenosine phosphorylase from Caldariella acidophila. 1. Purification and partial characterization].
作者信息
Oliva A, Cartenì-Farina M, Napolitano G, Romeo G, Zappia V, De Rosa M, Gambacorta A
出版信息
Boll Soc Ital Biol Sper. 1978 Dec 15;54(23):2355-61.
5'-Methylthioadenosine phosphorylase has been isolated from C.acidophila, a thermophilic bacterium living in acid hot springs at temperatures ranging from 63 to 89 degrees C. The enzyme has been purified to homogeneity in 32% yield. The enzyme shows a high degree of thermophilicity, its temperature optimum being 93 degrees C in the in vitro assay. The enzyme is exceptionally stable; no loss of activity was observable after exposure for 1 h at 100 degrees C. The optimum pH is about 7,2, with one-half of the maximal activity occurring at pH 6 and 9. The apparent Km for the substrates are: 8,3 x 10(-5) M for MTA and 4,3 x 10(-4) M for phosphate ions.
5'-甲硫基腺苷磷酸化酶已从嗜酸栖热菌中分离出来，嗜酸栖热菌是一种生活在温度范围为63至89摄氏度的酸性温泉中的嗜热细菌。该酶已被纯化至同质，产率为32%。该酶表现出高度嗜热性，其在体外测定中的最适温度为93摄氏度。该酶异常稳定；在100摄氏度下暴露1小时后未观察到活性丧失。最适pH约为7.2，在pH 6和9时活性为最大活性的一半。底物的表观Km值分别为：MTA为8.3×10⁻⁵ M，磷酸根离子为4.3×10⁻⁴ M。
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