Vargaftig B B
Agents Actions Suppl. 1977;2:9-39. doi: 10.1007/978-3-0348-7177-8_2.
Carrageenan or thrombin-induced aggregation of plasma-free rabbit platelets was inhibited by calcium and magnesium chelating agents, by N-ethylmaleimide and by drugs that increase the intra-cellular cyclic AMP content. Inhibitors of prostaglandin (PG) synthetase were only partially active, and had to be present in the platelet suspension to inhibit aggregation. Inhibition of PG synthetase, as evaluated by bioassay and by AA-induced platelet aggregation, was not reduced when inhibitors were washed from platelets. The phospholipase A2 inhibitors bromophenacyl bromide and mepacrine, the chymotrypsin inhibitor tosylphenylalaninechloromethylketone, catalase and dithiothreitol also inhibited aggregation, whereas inhibitors of trypsin failed to do so. Incubation of rabbit platelet-rich plasma with carrageenan was followed by generation of PG-like and of rabbit aorta contracting activities. Generation of these activities was inhibited by drugs effective against aggregation, and also by non-steroidal anti-inflammatory drugs. Aggregation of rabbit platelets by carrageenan and by thrombin does not appear to be dependent upon activation of PG synthetase, although PG-like substances are formed during aggregation.
角叉菜胶或凝血酶诱导的无血浆兔血小板聚集受到钙和镁螯合剂、N-乙基马来酰胺以及增加细胞内环磷酸腺苷含量的药物的抑制。前列腺素（PG）合成酶抑制剂仅具有部分活性，且必须存在于血小板悬液中才能抑制聚集。当从血小板中洗去抑制剂时，通过生物测定法和花生四烯酸诱导的血小板聚集评估的PG合成酶抑制作用并未降低。磷脂酶A2抑制剂溴苯甲酰溴和米帕林、胰凝乳蛋白酶抑制剂甲苯磺酰苯丙氨酸氯甲基酮、过氧化氢酶和二硫苏糖醇也抑制聚集，而胰蛋白酶抑制剂则不能。用角叉菜胶孵育富含兔血小板的血浆后，会产生类PG活性和兔主动脉收缩活性。这些活性的产生受到有效对抗聚集的药物以及非甾体抗炎药的抑制。角叉菜胶和凝血酶诱导的兔血小板聚集似乎不依赖于PG合成酶的激活，尽管在聚集过程中会形成类PG物质。
