Preparation of vertebrate photoreceptor membranes for study of adenylate cyclase, guanylate cyclase, and cyclic nucleotide phosphodiesterase.
作者信息
Keirns J J, Miki N, Bitensky M W
出版信息
Methods Enzymol. 1974;38:153-5. doi: 10.1016/0076-6879(74)38023-8.
PMID:4156068
Abstract
摘要
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The enzymatic preparation of [alpha-32P]ATP, [alpha-32P]GTP, [32P]cAMP, and [32P]cGMP, and their use in the assay of adenylate and guanylate cyclases and cyclic nucleotide phosphodiesterases.[α-32P]ATP、[α-32P]GTP、[32P]cAMP和[32P]cGMP的酶促制备及其在腺苷酸环化酶、鸟苷酸环化酶和环核苷酸磷酸二酯酶测定中的应用。
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Cyclic nucleotides and platelet aggregation. Effect of aggregating agents on the activity of cyclic nucleotide-metabolizing enzymes.环核苷酸与血小板聚集。聚集剂对环核苷酸代谢酶活性的影响。
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Improved two-step method for the assay of adenylate and guanylate cyclase.
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