Shukla O P
Indian J Biochem Biophys. 1974 Sep;11(3):192-200.
An organism, which degrades alpha-picoline but also utilizes 2-ethylpyridine or piperidine as alternative growth substrates, has been isolated from soil and characterized as arthrobacter sp. alpha-picoline-grown cells oxidize 2-ethylpyridine and vice versa. Other pyridine derivatives tested are neither utilized as growth substrates nor oxidized by the organism. alpha-Picolinate and 2-hydroxy-6-methylpyridine are not metabolized, indicating that degradation is neither initiated by methyl oxidation nor by hydroxylation in the 6-position of pyridine ring. Succinate semi-aldehyde and pyruvate accumulate when alpha-picoline oxidation by resting cell suspensions is blocked by semicarbazide. The Arthrobacter grown on alpha-picoline rapidly oxidizes succinate semi aldehyde...
从土壤中分离出一种既能降解α-甲基吡啶，又能利用2-乙基吡啶或哌啶作为替代生长底物的微生物，经鉴定为节杆菌属。以α-甲基吡啶培养的细胞能氧化2-乙基吡啶，反之亦然。所测试的其他吡啶衍生物既不能用作生长底物，也不能被该微生物氧化。α-吡啶甲酸盐和2-羟基-6-甲基吡啶不能被代谢，这表明降解既不是由甲基氧化引发，也不是由吡啶环6位的羟基化引发。当氨基脲阻断静止细胞悬液对α-甲基吡啶的氧化时，琥珀酸半醛和丙酮酸会积累。在α-甲基吡啶上生长的节杆菌能迅速氧化琥珀酸半醛……
