Petersen L C
Biochim Biophys Acta. 1979 Dec 6;548(3):636-41. doi: 10.1016/0005-2728(79)90070-7.
The effect of pH on the oxygen kinetics of cytochrome c oxidase incorporated into phospholipid vesicles is studied. The pH profiles of the oxygen kinetics of energized and deenergized oxidase vesicles are similar. An effect of pH on the slope of the reciprocal plot of rate against oxygen concentration is observed, and this may indicate that protons are involved in the rate limiting step of the reaction between oxygen and reduced oxidase. In contrast to the pH dependence of the oxygen kinetics, the binding of CO to the oxidase is not pH dependent.
研究了pH对掺入磷脂囊泡中的细胞色素c氧化酶氧动力学的影响。通电和断电的氧化酶囊泡的氧动力学pH曲线相似。观察到pH对速率与氧浓度倒数图斜率的影响，这可能表明质子参与了氧与还原型氧化酶反应的限速步骤。与氧动力学的pH依赖性相反，CO与氧化酶的结合不依赖于pH。
