[癸酸，一种用于植物亚细胞组分体外生物合成油酸的新前体]

[Decanoic acid, new precursor for in vitro biosynthesis of oleic acid by a plant subcellular fraction].

作者信息

Mazliak P, Grosbois M, Decotte A M

出版信息

Biochimie. 1975;57(8):943-52. doi: 10.1016/s0300-9084(75)80216-1.

Abstract

Various membraneous fractions prepared from a cauliflower homogenate synthesize radioactive oleic acid when they are incubated in a 14C-decanolate solution. The more active fraction is formed of vesicles sedimenting at 30,000 g x 20 mn (heavy microsomes). The labelled precursor is transformed by this fraction mainly into oleic acid and hydroxyacids. ATP, NADPH, CoA and oxygen are required for these reactions. Labelled fatty acids, longer than lauric and (i.e. 14C-myristic, 14C-palmitic and 14C-stearic acids) are not transformed into oleic acid by the subcellular fraction studied in this paper.

摘要

从花椰菜匀浆制备的各种膜组分在14C - 癸醇溶液中孵育时会合成放射性油酸。活性较高的组分由在30,000 g×20分钟沉降的囊泡组成（重微粒体）。该组分将标记的前体主要转化为油酸和羟基酸。这些反应需要ATP、NADPH、辅酶A和氧气。本文研究的亚细胞组分不会将比月桂酸更长的标记脂肪酸（即14C - 肉豆蔻酸、14C - 棕榈酸和14C - 硬脂酸）转化为油酸。