从干燥拭子中分离白喉棒状杆菌的改良培养技术。
Modified culture technique for Corynebacterium diphtheriae isolation from desiccated swabs.
作者信息
Facklam R R, Lawrence D N, Sottnek F O
出版信息
J Clin Microbiol. 1978 Feb;7(2):137-8. doi: 10.1128/jcm.7.2.137-138.1978.
Abstract
Corynebacterium diphtheriae was isolated from pyoderma and ulcerative skin lesions with a modified delayed culture procedure as late as 9 weeks after field collection of silica gel-desiccated swabs. Biotypes gravis and mitis were identified. Most isolates were nontoxigenic. Todd-Hewitt broth enrichment enhanced recovery of C. diphtheriae by 70%.
摘要
采用改良的延迟培养程序，在硅胶干燥拭子现场采集9周后，从脓皮病和溃疡性皮肤病变中分离出白喉棒状杆菌。鉴定出了重型和轻型生物型。大多数分离株不产毒素。托德-休伊特肉汤增菌使白喉棒状杆菌的回收率提高了70%。
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