从干燥拭子中分离白喉棒状杆菌的改良培养技术。
Modified culture technique for Corynebacterium diphtheriae isolation from desiccated swabs.
作者信息
Facklam R R, Lawrence D N, Sottnek F O
出版信息
J Clin Microbiol. 1978 Feb;7(2):137-8. doi: 10.1128/jcm.7.2.137-138.1978.
Abstract
Corynebacterium diphtheriae was isolated from pyoderma and ulcerative skin lesions with a modified delayed culture procedure as late as 9 weeks after field collection of silica gel-desiccated swabs. Biotypes gravis and mitis were identified. Most isolates were nontoxigenic. Todd-Hewitt broth enrichment enhanced recovery of C. diphtheriae by 70%.
摘要
采用改良的延迟培养程序，在硅胶干燥拭子现场采集9周后，从脓皮病和溃疡性皮肤病变中分离出白喉棒状杆菌。鉴定出了重型和轻型生物型。大多数分离株不产毒素。托德-休伊特肉汤增菌使白喉棒状杆菌的回收率提高了70%。
相似文献
1
Modified culture technique for Corynebacterium diphtheriae isolation from desiccated swabs.
J Clin Microbiol. 1978 Feb;7(2):137-8. doi: 10.1128/jcm.7.2.137-138.1978.
2
Epidemiologic studies among Amerindian populations of Amazônia. I. Pyoderma: prevalence and associated pathogens.
Am J Trop Med Hyg. 1979 May;28(3):548-58.
3
Non-toxigenic Corynebacterium diphtheriae biovar gravis: evidence for an invasive clone in a south-eastern Australian community.
Med J Aust. 1996 Jan 15;164(2):72-5. doi: 10.5694/j.1326-5377.1996.tb101351.x.
4
Prevalence of Corynebacterium diphtheriae in pyoderma and other skin lesions.
Indian J Pathol Microbiol. 1979 Jan;22(1):37-45.
5
Polyacrylamide gel electrophoresis of Corynebacterium diphtheriae: a possible epidemiological aid.
Appl Microbiol. 1971 Nov;22(5):885-90. doi: 10.1128/am.22.5.885-890.1971.
6
Emergence of related nontoxigenic Corynebacterium diphtheriae biotype mitis strains in Western Europe.
Emerg Infect Dis. 1999 May-Jun;5(3):477-80. doi: 10.3201/eid0503.990326.
7
Changes in MLST profiles and biotypes of Corynebacterium diphtheriae isolates from the diphtheria outbreak period to the period of invasive infections caused by nontoxigenic strains in Poland (1950-2016).
BMC Infect Dis. 2018 Mar 9;18(1):121. doi: 10.1186/s12879-018-3020-1.
8
[Cutaneous leishmaniotic ulcers with Corynebacterium diphtheriae].
Rev Soc Bras Med Trop. 2002 Jul-Aug;35(4):311-3. doi: 10.1590/s0037-86822002000400006.
9
Incidence of cutaneous diphtheria in Bangladesh.
Bangladesh Med Res Counc Bull. 1983 Dec;9(2):49-53.
10
[Isolation of Corynebacterium diphtheriae subsp. belfanti from a cow with chronic active dermatitis].
Schweiz Arch Tierheilkd. 1996;138(12):596-9.
引用本文的文献
1
Silica desiccant packets for storage and transport of Streptococcus pneumoniae and other clinically relevant species.
PLoS One. 2013 Aug 7;8(8):e72353. doi: 10.1371/journal.pone.0072353. Print 2013.
2
Use of silica desiccant packets for specimen storage and transport to evaluate pneumococcal nasopharyngeal carriage among Nepalese children.
J Clin Microbiol. 2008 Sep;46(9):3175-6. doi: 10.1128/JCM.00906-08. Epub 2008 Jul 2.
3
Evaluation of silica gel packages for transport of Neisseria meningitidis.
J Clin Microbiol. 1998 Jun;36(6):1765-6. doi: 10.1128/JCM.36.6.1765-1766.1998.
4
Silica gel as transport medium for Corynebacterium diphtheriae under tropical conditions (Indonesia ).
J Clin Microbiol. 1987 May;25(5):964-5. doi: 10.1128/jcm.25.5.964-965.1987.
本文引用的文献
1
A study of a modified Tinsdale's medium for the primary isolation of Corynebacterium diphtheriae.
J Infect Dis. 1958 Jan-Feb;102(1):88-93. doi: 10.1093/infdis/102.1.88.
2
Tissue culture method for toxigenicity testing of Corynebacterium diphtheriae.
Appl Microbiol. 1968 Nov;16(11):1748-52. doi: 10.1128/am.16.11.1748-1752.1968.
3
Tissue culture method for the titration of diphtheria antitoxin in human sera.
Health Lab Sci. 1967 Jul;4(3):181-8.
4
Letter: Prevalence of streptococci and staphylococci in lesions of impetigo.
Br J Dermatol. 1974 Jul;91(1):115-6. doi: 10.1111/j.1365-2133.1974.tb06728.x.
5
Problems in the use of the in vitro toxigenicity test for Corynebacterium diphtheriae.
Am J Clin Pathol. 1972 Feb;57(2):244-6. doi: 10.1093/ajcp/57.2.244.
6
Value of desiccated swabs for streptococcal epidemiology in the field.
Appl Microbiol. 1973 Jan;25(1):135-8. doi: 10.1128/am.25.1.135-138.1973.
7
Silica gel as a transport medium for Corynebacterium diphtheriae.
South Med J. 1972 Nov;65(11):1383-4. doi: 10.1097/00007611-197211000-00020.
Zotero 插件