Stenzel K H, Rubin A L
Science. 1966 Jul 29;153(3735):537-9. doi: 10.1126/science.153.3735.537.
Incorporation of C(14)-amino acids into high-molecular-weight material precipitable by trichloroacetic acid indicates that microsomal cell-free systems, derived from spleens of immunized rabbits, are active in protein synthesis. Protein was made soluble by ultrasonic irradiation of the cell-free incubation mixtures, and low-molecular-weight materials were removed by dialysis and gel filtration. Chromatography and radio-immunoelectrophoresis of this soluble protein fraction reveal C(14)-labeled protein having several characteristics of gamma globulin.
将C(14) -氨基酸掺入可被三氯乙酸沉淀的高分子量物质中，这表明源自免疫兔脾脏的微粒体无细胞系统在蛋白质合成中具有活性。通过对无细胞孵育混合物进行超声辐照使蛋白质溶解，并通过透析和凝胶过滤去除低分子量物质。对该可溶性蛋白质部分进行色谱分析和放射免疫电泳，结果显示C(14)标记的蛋白质具有γ球蛋白的若干特性。
