Reid R T, Minden P, Farr R S
J Exp Med. 1966 May 1;123(5):845-58. doi: 10.1084/jem.123.5.845.
Five human sera with reaginic activity to a number of allergens were fractionated using anion exchange chromatography. In each serum, fractions which contained detectable IgG and no detectable IgA had capacity to fix to skin and subsequently elicit a P-K reaction. Four of these sera had reaginic activity about equally distributed between fractions containing only IgG and fractions containing mixtures of IgG and IgA. A fifth serum contained reaginic activity to crystalline bovine serum albumin (BSA) and most of the activity was associated with the fraction which contained only IgG. This serum was extensively studied using a variety of techniques and it was confirmed that most of the reagin to BSA in this serum was in those fractions containing only IgG. Since reaginic activity can no longer be considered a unique property of IgA the implications of finding antibody with reaginic qualities in immunoglobulins other than IgA are discussed.
使用阴离子交换色谱法对五份对多种变应原具有反应素活性的人血清进行了分离。在每份血清中，含有可检测到的IgG且未检测到IgA的组分具有固定于皮肤并随后引发P-K反应的能力。其中四份血清的反应素活性在仅含IgG的组分和含IgG与IgA混合物的组分之间大致均匀分布。第五份血清对结晶牛血清白蛋白（BSA）具有反应素活性，且大部分活性与仅含IgG的组分相关。使用多种技术对该血清进行了广泛研究，并证实该血清中针对BSA的大部分反应素存在于仅含IgG的那些组分中。由于反应素活性不再被认为是IgA的独特特性，因此讨论了在IgA以外的免疫球蛋白中发现具有反应素性质的抗体的意义。
