人体组织在体外形成免疫球蛋白。II. 定量研究。
The formation of immunoglobulins by human tissues in vitro. II. Quantitative studies.
作者信息
van Furth R
出版信息
Immunology. 1966 Jul;11(1):13-8.
Abstract
The present paper deals with some of the quantitative aspects of immunoglobulin formation . The results of this study show that isolated spleen cells synthesize about 1.25 μg IgG per 10 lymphoid cells during the first 6 hours of incubation. The initial rate of synthesis was not maintained throughout the 48 hours of incubation, but decreased markedly after 6 hours. Comparison of the intensity of the autoradiographic line with the amount of radioactive immunoglobulin in the precipitation line revealed that a very distinct line (gradation +++) corresponds to about 0.0125 μg IgG. The limit of visibility (+) is reached at approximately 0.0002 μg radioactive IgG.
摘要
本文探讨了免疫球蛋白形成的一些定量方面。本研究结果表明，在培养的最初6小时内，分离的脾细胞每10个淋巴细胞合成约1.25μg IgG。在整个48小时的培养过程中，合成的初始速率并未保持，而是在6小时后显著下降。将放射自显影片条带的强度与沉淀线中放射性免疫球蛋白的量进行比较，发现一条非常清晰的条带（灰度+++）对应约0.0125μg IgG。在放射性IgG约为0.0002μg时达到可见极限（+）。
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2
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