人白细胞干扰素对多聚核糖核苷酸的亲和力。

Affinity of human leukocyte interferon for polyribonucleotides.

作者信息

Chadha K C, Grob P M

出版信息

Cell Biol Int Rep. 1979 Nov;3(8):663-9. doi: 10.1016/0309-1651(79)90097-3.

Abstract

Human leukocyte interferon (HL-IF)binds to AGPOLY(A)TM, AGPOLY(U)TM and AGPOLY(I)TM. The bound interferon could be displaced from all three polyribonucleotides by including sodium chloride in the eluant. The nature of interaction of HL-IF with polyribonucleotides is electrostatic and not hydrophobic since its binding was not prevented in the presence of 50% ethylene glycol. The binding of HL-IF on AGPOLY(I)TM is stronger at lower pH since an increase in ionic strength is required to displace it.

摘要

人白细胞干扰素（HL-IF）与AGPOLY(A)TM、AGPOLY(U)TM和AGPOLY(I)TM结合。通过在洗脱液中加入氯化钠，结合的干扰素可从所有三种多聚核糖核苷酸上被置换下来。HL-IF与多聚核糖核苷酸的相互作用性质是静电作用而非疏水作用，因为在50%乙二醇存在的情况下其结合并未受到抑制。在较低pH值下，HL-IF在AGPOLY(I)TM上的结合更强，因为需要增加离子强度才能将其置换下来。