一种基于噻唑基蓝还原反应的试剂配方中黄递酶活性的新检测方法。
A new assay for diaphorase activity in reagent formulations, based on the reduction of thiazolyl blue.
作者信息
Boethling R S, Weaver T L
出版信息
Clin Chem. 1979 Dec;25(12):2040-2.
PMID:41650
Abstract
This new assay procedure for diaphorase eliminates problems of high blank rates and nonlinear kinetics associated with other methods. The dye thiazolyl blue tetrazolium bromide is reduced in the presence of NADH and diaphorase to yield a colored formazan, which as maximum absorbance at 560 nm.
摘要
这种新的黄递酶检测方法消除了与其他方法相关的高空白率和非线性动力学问题。在NADH和黄递酶存在的情况下，噻唑蓝溴化四氮唑染料被还原，生成一种在560nm处有最大吸光度的有色甲臜。
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