Liu Yong-Qing, An Bo-Wen, Jiang Rui-Yong, Huang Chan, Cai Su-Qian, Zhang Bi-Xiao, Leng Yan-Li, Pan Jing-Zhen, Liang Le, Cai Xiao-Hua
School of Chemical Engineering, Guizhou Minzu University, Guiyang 550025, China.
School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China.
J Colloid Interface Sci. 2026 Feb 6;711:140064. doi: 10.1016/j.jcis.2026.140064.
Rapid and intelligent detection of bioactive molecules and metal ions is vital for health and environmental safety. Herein, a dual-functional nitrogen-doped carbon dots (N-CDs) for L-Histidine (L-His) and Al detection was developed employing chitosan (CTS) and umbelliferone (UMB) as the substrates. The N-CDs exhibited a distinct "turn-on" fluorescence response to L-His and a subsequent signal recovery towards Al with low detection limits (LODs) of 0.950 μM and 4.393 μM. The underlying sensing mechanisms were rigorously elucidated via density functional theory (DFT) calculations and independent gradient model based on Hirshfeld partition (IGMH) analyses, highlighting the pivotal role of surface functional groups. The practical applicants of N-CDs were demonstrated through the fabrication of fluorescent hydrogels, solid-state films for light-emitting diodes (LEDs), and anti-counterfeiting patterns. Notably, a portable and intelligent sensing platform driven by STM32 microcontroller was engineered to capture and analyze RGB signals to realize smart and on-site quantification of L-His, which provided a robust and cost-effective strategy for real-time monitoring of analytes in complex matrices.
快速、智能地检测生物活性分子和金属离子对健康和环境安全至关重要。在此，以壳聚糖（CTS）和伞形酮（UMB）为底物，开发了一种用于检测L-组氨酸（L-His）和铝的双功能氮掺杂碳点（N-CDs）。N-CDs对L-His表现出明显的“开启”荧光响应，随后对铝呈现信号恢复，检测限低至0.950 μM和4.393 μM。通过密度泛函理论（DFT）计算和基于Hirshfeld划分的独立梯度模型（IGMH）分析，严格阐明了潜在的传感机制，突出了表面官能团的关键作用。通过制备荧光水凝胶、发光二极管（LED）固态膜和防伪图案，展示了N-CDs的实际应用。值得注意的是，设计了一个由STM32微控制器驱动的便携式智能传感平台，用于捕获和分析RGB信号，以实现L-His的智能现场定量，为复杂基质中分析物的实时监测提供了一种强大且经济高效的策略。
