采用混合血细胞吸附技术对系统性红斑狼疮和类风湿性关节炎患者血清中可溶性核抗原抗体的发生率进行评估。
The incidence of antibodies to a soluble nuclear antigen in the sera of patients with systemic lupus erythematosus and rheumatoid arthritis estimated with the mixed haemadsorption technique.
作者信息
Jonsson J
出版信息
Clin Exp Immunol. 1967 Jul;2(4):415-26.
The incidence of antibodies to a `soluble' nuclear antigen in sera from cases of connective tissue disease was studied with the mixed haemadsorption technique, which was more sensitive than the indirect immunofluorescent test and more accurate for quantitative determinations. The results with unabsorbed sera were not affected by the coexistence of antibodies to nucleoprotein and desoxyribonucleic acid (DNA). Antibodies to soluble nuclear antigen were four times more common in sera from cases of systemic lupus erythematosus (SLE) than in sera from cases of rheumatoid arthritis, where they were about as frequent as in sera from healthy male blood donors. However, their incidence in SLE was 50% and thus not high enough to make them useful as an independent diagnostic criterion. The antigenicity of the soluble nuclear material was preserved after treatment with acetone but not with ethanol.
采用混合血细胞吸附技术研究结缔组织病患者血清中针对“可溶性”核抗原的抗体发生率，该技术比间接免疫荧光试验更敏感，在定量测定方面更准确。未吸收血清的检测结果不受核蛋白和脱氧核糖核酸（DNA）抗体共存的影响。系统性红斑狼疮（SLE）患者血清中针对可溶性核抗原的抗体比类风湿性关节炎患者血清中的抗体常见四倍，而类风湿性关节炎患者血清中该抗体的频率与健康男性献血者血清中的频率大致相同。然而，其在SLE中的发生率为50%，因此不足以作为独立的诊断标准。用丙酮处理后，可溶性核物质的抗原性得以保留，但用乙醇处理则不然。
Zotero 插件