通过β-半乳糖苷酶活性测定乙二胺四乙酸和磷脂酶对大肠杆菌的裂解作用。
Lysis of Escherichia coli by ethylenediaminetetraacetate and phospholipases as measured by beta-galactosidase activity.
作者信息
Slein M W, Logan G F
出版信息
J Bacteriol. 1967 Oct;94(4):934-41. doi: 10.1128/jb.94.4.934-941.1967.
Abstract
A permeaseless mutant of Escherichia coli, which produces beta-galactosidase constitutively, was treated briefly with ethylenediaminetetraacetate and then with the phospholipases of Bacillus cereus. Cell lysis occurred, as indicated by an increase in beta-galactosidase activity and a decrease in absorbancy of the cell suspension. The susceptibility of the cells to attack by ethylenediaminetetraacetate and the phospholipases was markedly affected by the age of the cells when harvested. The results suggest that permeability changes may be associated with the activity of a phospholipase that specifically degrades phosphatidyl ethanolamine. A sonic-treatment method for determining the total beta-galactosidase content of E. coli cells, which is independent of their age when harvested, is described.
摘要
一种组成型产生β-半乳糖苷酶的大肠杆菌无渗透突变体，先用乙二胺四乙酸短暂处理，然后用蜡状芽孢杆菌的磷脂酶处理。如β-半乳糖苷酶活性增加和细胞悬浮液吸光度降低所示，细胞发生了裂解。收获时细胞的年龄对细胞受乙二胺四乙酸和磷脂酶攻击的敏感性有显著影响。结果表明，通透性变化可能与一种特异性降解磷脂酰乙醇胺的磷脂酶的活性有关。本文描述了一种用于测定大肠杆菌细胞总β-半乳糖苷酶含量的超声处理方法，该方法与收获时细胞的年龄无关。
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