Zafar Asma, Rahman Ziaur, Kiran Sehrish, Aftab Muhammad Nauman, Maqsood Neha, Shafique Eeza
Faculty of Science and Technology, University of Central Punjab, Lahore, Pakistan.
Institute of Industrial Biotechnology, Government College University, Lahore, Pakistan.
Prep Biochem Biotechnol. 2026 Feb 14:1-11. doi: 10.1080/10826068.2026.2631148.
Microbial amylases play an important role in several industrial processes, yet improving production efficiency and enzyme stability remains an ongoing challenge. The present study evaluates a co-cultivation approach using and to enhance α-amylase production under solid-state fermentation using rice husk as an economical substrate. Compared to monoculture systems, co-cultivation resulted in consistently higher enzyme production under all optimized conditions, suggesting a synergistic interaction between the two fungi. Optimal production was achieved at pH 5.0, 30 °C, and 72 hr of incubation, with maximum activity observed at a substrate concentration of 1.5% and 60 °C. The produced α-amylase exhibited stability over a broad range of pH and temperature conditions, retaining 95.84% activity at pH 6.0 after 3 hr and 45.5% activity after 4 hr at 80 °C. Activity was enhanced by Ca, Mg, and Cu ions, while EDTA caused partial inhibition, indicating metal ion involvement in catalysis. The enzyme also showed tolerance to organic solvents, although detergent exposure reduced activity at higher concentrations. Overall, the results suggest that co-cultivation can improve α-amylase production while yielding an enzyme with properties relevant for industrial use.
微生物淀粉酶在多个工业过程中发挥着重要作用，但提高生产效率和酶的稳定性仍然是一个持续存在的挑战。本研究评估了一种使用[具体菌种1]和[具体菌种2]的共培养方法，以在以稻壳为经济底物的固态发酵条件下提高α-淀粉酶的产量。与单一培养系统相比，在所有优化条件下，共培养产生的酶产量始终更高，这表明两种真菌之间存在协同相互作用。在pH 5.0、30°C和培养72小时的条件下实现了最佳产量，在底物浓度为1.5%和60°C时观察到最大活性。所产生的α-淀粉酶在广泛的pH和温度条件下表现出稳定性，在pH 6.0下3小时后保留95.84%的活性，在80°C下4小时后保留45.5%的活性。Ca、Mg和Cu离子增强了活性，而EDTA导致部分抑制，表明金属离子参与催化。该酶对有机溶剂也表现出耐受性，尽管在较高浓度的洗涤剂作用下活性会降低。总体而言，结果表明共培养可以提高α-淀粉酶的产量，同时产生具有与工业用途相关特性的酶。
