Chirigos M A, Stylos W A, Schultz R M, Fullen J R
Cancer Res. 1978 Apr;38(4):1085-91.
With an L1210 tumor vaccine model, three biological and two chemical agents were tested for their ability to act as adjuvants. Adjuvant was administered with irradiated L1210 cells to immunize mice against this poorly immunogenic tumor. Two chemicals, pyran copolymer and glucan, and one biological, Brucella abortus strain 456 ether extract, were shown to be strong stimulators of antitumor immunity. Vaccination with irradiated tumor cells or adjuvant alone did not produce host resistance. Optimal immunity to challenge was produced by concomitant administration of either pyran copolymer, glucan, or B. abortus strain 456 ether extract with L1210 vaccine. Antitumor immunity was maximally expressed when vaccine and adjuvant were administered i.p. Evidence for systemic immunity was demonstrated when challenge was at a distal s.c. site. Mice immune to challenge were found to be refractory to a later rechallenge.
在L1210肿瘤疫苗模型中，测试了三种生物制剂和两种化学制剂作为佐剂的能力。将佐剂与经辐射的L1210细胞一起给药，以免疫小鼠对抗这种免疫原性较差的肿瘤。两种化学物质，即聚吡喃共聚物和葡聚糖，以及一种生物制剂，流产布鲁氏菌456株乙醚提取物，被证明是抗肿瘤免疫的强刺激剂。单独用经辐射的肿瘤细胞或佐剂进行疫苗接种不会产生宿主抗性。通过将聚吡喃共聚物、葡聚糖或流产布鲁氏菌456株乙醚提取物与L1210疫苗同时给药，可产生对攻击的最佳免疫力。当疫苗和佐剂经腹腔注射给药时，抗肿瘤免疫力得到最大程度的表达。当在远端皮下部位进行攻击时，证明了全身免疫的证据。发现对攻击具有免疫力的小鼠对随后的再次攻击具有抗性。
