藻胆蛋白中发色团与蛋白质的连接，VII. 来自假鱼腥藻W 1173和嗜鱼席藻的C-藻红蛋白发色团区域的氨基酸序列

On the linkages between chromophore and protein in biliproteins, VII. Amino acid sequence in the chromophore regions of C-phycoerythrin from Pseudanabaena W 1173 and Phormidium persicinum.

作者信息

Muckle G, Otto J, Rüdiger W

出版信息

Hoppe Seylers Z Physiol Chem. 1978 Mar;359(3):345-55.

Abstract

Bilipeptides from all chromophore regions were prepared by trypsin digestion of C-phycoerythrin from Pseudanabaena W 1173 and Phormidium persicinum. Analytical separation and quantitative determination of bilipeptides was achieved by isoelectric focusing, preparative isolation by gel chromatography and ion-exchange chromatography. Amino acid analysis revealed cysteine as the only amino acid common to all chromopeptides. Amino acid sequences were determined by Edman degradation and the dansyl-Edman technique. Sequences are different in all 5 and 6 chromophore regions, respectively. Possible homologies are discussed. A thioether linkage between ring A of the chromophore and cysteine was found in the bilipeptides (as before in biliproteins). A second linkage (serine ester) was found in only one peptide (CM 4.I from Pseudanabaena W 1173). This peptide absorbs as cation at a longer wavelength (559 nm) than the other bilipeptides (542 - 550 nm).

摘要

通过胰蛋白酶消化来自伪鱼腥藻W 1173和嗜鱼席藻的C-藻红蛋白，制备了来自所有发色团区域的胆肽。通过等电聚焦实现胆肽的分析分离和定量测定，通过凝胶色谱和离子交换色谱进行制备分离。氨基酸分析表明，半胱氨酸是所有发色肽共有的唯一氨基酸。通过埃德曼降解法和丹磺酰-埃德曼技术测定氨基酸序列。在所有5个和6个发色团区域中，序列分别不同。讨论了可能的同源性。在胆肽中发现了发色团A环与半胱氨酸之间的硫醚键（如之前在胆蛋白中一样）。仅在一种肽（来自伪鱼腥藻W 1173的CM 4.I）中发现了第二种键（丝氨酸酯）。该肽作为阳离子在比其他胆肽更长的波长（559 nm）处吸收（542 - 550 nm）。