D-甘油酸2,3-二磷酸与脱氧血红蛋白和氧合血红蛋白结合的pH依赖性。氧合血红蛋白中结合位点数量的测定。
The pH dependence of the binding of D-glycerate 2,3-bisphosphate to deoxyhemoglobin and oxyhemoglobin. Determination of the number of binding sites in oxyhemoglobin.
作者信息
Van Beek G G, De Bruin S H
出版信息
Eur J Biochem. 1979 Oct 15;100(2):497-502. doi: 10.1111/j.1432-1033.1979.tb04194.x.
The number of Bohr protons released upon oxygenation has been measured over a large range of human hemoglobin concentrations (0.02 to 4.5 mM) in the presence of equimolar amounts of D-glycerate 2,3-bisphosphate. From these data the association constants for the binding of this organic phosphate to deoxyhemoglobin and oxyhemoglobin were calculated at different pH values. The maximum number of protons absorbed upon binding to oxyhemoglobin was determined as well. The maximum number of protons bound to deoxyhemoglobin upon binding of D-glycerate 2,3-bisphosphate was measured independently. From the pH dependence of the association constants and the maximum number of protons absorbed it could be concluded that only one D-glycerate 2,3-bisphosphate can be bound to both deoxyhemoglobin and oxyhemoglobin.
在等摩尔量的2,3-二磷酸甘油酸存在下，在很宽的人血红蛋白浓度范围（0.02至4.5 mM）内测量了氧合时释放的玻尔质子数量。根据这些数据，计算了该有机磷酸盐在不同pH值下与脱氧血红蛋白和氧合血红蛋白结合的缔合常数。还确定了与氧合血红蛋白结合时吸收的最大质子数。独立测量了2,3-二磷酸甘油酸与脱氧血红蛋白结合时与之结合的最大质子数。从缔合常数的pH依赖性和吸收的最大质子数可以得出结论，只有一个2,3-二磷酸甘油酸可以与脱氧血红蛋白和氧合血红蛋白结合。
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