Seed J L, Boff M, Bennett J L
J Parasitol. 1978 Apr;64(2):283-9.
A biochemical methods has been developed for detecting phenol oxidase in female Schistosoma mansoni. Enzyme activity is observed only after incubation of the female schistosomes for an extended period of time in tissue culture media. Male S. mansoni do not contain detectable levels of phenol oxidase activity. The properties of this enzyme are similar to those identified for a phenol oxidase from Fasciola hepatica. L-DOPA, dopamine, and tyrosine were found to be good substrates for this enzyme. Vmax = 14.1, 8.1, and 6.1 mumoles O2/min/mg protein for each substrate, respectively. This enzyme appears to be associated with egg production and thus may be a useful marker for biochemical and immunological studies.
已开发出一种生化方法来检测曼氏血吸虫雌虫中的酚氧化酶。只有在将雌虫在组织培养基中长时间孵育后才能观察到酶活性。曼氏血吸虫雄虫不含可检测水平的酚氧化酶活性。这种酶的特性与从肝片吸虫中鉴定出的酚氧化酶相似。发现L - 多巴、多巴胺和酪氨酸是这种酶的良好底物。每种底物的Vmax分别为14.1、8.1和6.1微摩尔O2/分钟/毫克蛋白质。这种酶似乎与产卵有关，因此可能是生化和免疫学研究的有用标志物。
