生物流体中三氟乙酸的气相色谱 - 质谱测定法。在氟烷代谢研究中的应用。
The gas chromatographic mass spectrometric determination of trifluoroacetic acid in biological fluid. Application to halothane metabolism.
作者信息
Karashima D, Takahashi S, Shigematsu A, Furukawa T, Kuhara T
出版信息
Biomed Mass Spectrom. 1976 Feb;3(1):41-4. doi: 10.1002/bms.1200030108.
The methyl ester of trifluoroacetic acid was prepared by reaction with N,N'-dimethylformamide dimethylacetal and was successfully passed through a gas chromatograph. Trifluoroacetic acid was detected by the use of gas chromatography and low and high resolution gas chromatography mass spectrometry in an acidic extract of an incubation medium containing microsomes, reduced nicotinamide adenine dinucleotide phosphate, oxygen and halothane. However, trifluoroacetic acid could not be detected when nicotinamide adenine dinucleotide or oxygen was omitted from the incubation system. From these results, it was proved that halothane is oxidatively metabolized to trifluoroacetic acid by hepatic microsomes.
三氟乙酸甲酯通过与N，N'-二甲基甲酰胺二甲基缩醛反应制备，并成功通过气相色谱仪。在含有微粒体、还原型烟酰胺腺嘌呤二核苷酸磷酸、氧气和氟烷的孵育培养基的酸性提取物中，使用气相色谱法以及低分辨率和高分辨率气相色谱-质谱联用技术检测到了三氟乙酸。然而，当孵育系统中省略烟酰胺腺嘌呤二核苷酸或氧气时，无法检测到三氟乙酸。从这些结果证明，氟烷被肝微粒体氧化代谢为三氟乙酸。
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