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用荧光抗体显色的沉淀带摄影。

Photography of precipitin bands developed with fluorescent antibodies.

作者信息

Hiramoto R N, Hamlin M

出版信息

Immunology. 1968 Jul;15(1):31-3.

PMID:4174145
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1409431/
Abstract

A procedure demonstrating the usefulness of combining fluorescence with agar diffusion is presented. A minor obstacle to its use is the means of permanently recording the colours that are obtained. The method described gives the conditions under which photographs can be taken. However, since the degree of fluorescence may vary from specimen to specimen exposure times, -openings and conditions of lighting must be varied for optimum results.

摘要

本文介绍了一种将荧光与琼脂扩散相结合的实用方法。该方法应用的一个小障碍是如何永久记录所获得的颜色。本文所述方法给出了拍摄照片的条件。然而,由于不同标本的荧光程度可能不同,因此必须改变曝光时间、光圈和光照条件以获得最佳效果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1409431/e3eb3024f919/immunology00390-0044-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1409431/496ab9ba0d50/immunology00390-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1409431/a7993ad63999/immunology00390-0044-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1409431/4c17ff58a7ef/immunology00390-0044-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1409431/e3eb3024f919/immunology00390-0044-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1409431/496ab9ba0d50/immunology00390-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1409431/a7993ad63999/immunology00390-0044-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1409431/4c17ff58a7ef/immunology00390-0044-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1409431/e3eb3024f919/immunology00390-0044-d.jpg

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引用本文的文献

1
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Infect Immun. 1973 Jun;7(6):981-91. doi: 10.1128/iai.7.6.981-991.1973.

本文引用的文献

1
[A micro-method of immuno-electrophoresis].[免疫电泳的微量方法]
Int Arch Allergy Appl Immunol. 1955;7(2):103-10.
2
Antigenic relationship of mouse rhabdomyosarcoma to human rhabdomyosarcoma and to human and mouse muscle.小鼠横纹肌肉瘤与人类横纹肌肉瘤以及人类和小鼠肌肉的抗原关系。
Cancer Res. 1966 Jul;26(7):1486-91.