Bercovich Z, Lagendijk W
Tijdschr Diergeneeskd. 1978 Apr 15;103(8):407-16.
The Milk Ring Test (MRT) can be used to detect Brucella antibodies in tank milk if the following points are considered: (1) Before testing, the milk must be stored at least 72 hours at 6 degrees C. (2) To obtain a reliable reaction one must use 8 ml milk and 0.8 ml antigen, instead of 1 ml milk and 0,05 ml antigen. (3) The 4 vol.% antigen appears to be more sensitive than the 5 vol.% antigen. (4) A standard negative milk sample should be included in a batch to be examined. It can help to determine doubtful results. (5) For further determination of a doubtful result obtained from the 1 ml milk reaction, 3 ml milk and 0.05 ml antigen can be used.
