Osunkoya B O, McFarlane H, Luzzatto L, Udeozo I O, Mottram F C, Williams A I, Ngu V A
Immunology. 1968 Jun;14(6):851-60.
(1) Freshly dispersed cells from four Burkitt's lymphoma biopsies, and three `lymphoblast' cell lines (OB2, OB3 and OB6) derived from the tumour were tested for capacity to synthesize immunoglobulins . Using labelled amino acid incorporation, electrophoretic, radio-immunoelectrophoretic and ultracentrifugation techniques, it was possible to demonstrate the release of labelled proteins with antigenic characteristics of immunoglobulins by all four biopsy samples, and the two cell lines tested by these techniques. Newly synthesized labelled proteins were demonstrable in the culture medium within 1 hour of incubation. In a separate series of experiments, absorption and immunodiffusion techniques were used in the characterization of proteins synthesized by two cell lines (OB2 and OB3) growing as healthy continuous cultures. (2) Freshly isolated cells from Burkitt's lymphoma are capable of immunoglobulin synthesis . Cells from all four fresh biopsy materials produced IgG. One cell line (OB2) produced IgG and type-κ light chains, while OB6 cell line produced IgA. Immunoglobulin synthesis was not detected in OB3 cell line by immunodiffusion technique. (3) Cells from each biopsy specimen or cell line produced not more than one type of immunoglobulin, although there was a wide variation in the sedimentation coefficient of the protein molecules synthesized by one (OB6) and presumably all other cell lines.
(1) 对取自4例伯基特淋巴瘤活检组织的新鲜分散细胞，以及源自该肿瘤的3个“淋巴母细胞”细胞系（OB2、OB3和OB6）进行了免疫球蛋白合成能力检测。采用标记氨基酸掺入、电泳、放射免疫电泳和超速离心技术，所有4个活检样本以及通过这些技术检测的2个细胞系均能释放出具有免疫球蛋白抗原特性的标记蛋白。孵育1小时内即可在培养基中检测到新合成的标记蛋白。在另一系列实验中，采用吸收和免疫扩散技术对以健康连续培养方式生长的2个细胞系（OB2和OB3）合成的蛋白质进行特性鉴定。(2) 新鲜分离的伯基特淋巴瘤细胞能够合成免疫球蛋白。所有4份新鲜活检材料的细胞均产生IgG。一个细胞系（OB2）产生IgG和κ型轻链，而OB6细胞系产生IgA。免疫扩散技术未在OB3细胞系中检测到免疫球蛋白合成。(3) 每个活检标本或细胞系的细胞产生的免疫球蛋白不超过一种类型，尽管由一个细胞系（OB6）以及可能所有其他细胞系合成的蛋白质分子沉降系数存在很大差异。
